Composite

Part:BBa_K3866031:Design

Designed by: Pericles Vasileiou   Group: iGEM21_Thessaly   (2021-09-24)


Propionate Production Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2948
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2948
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2948
    Illegal BglII site found at 8379
    Illegal BamHI site found at 1148
    Illegal BamHI site found at 2236
    Illegal BamHI site found at 4872
    Illegal BamHI site found at 5148
    Illegal BamHI site found at 7067
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2948
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2948
    Illegal NgoMIV site found at 3263
    Illegal AgeI site found at 983
    Illegal AgeI site found at 1400
    Illegal AgeI site found at 4707
    Illegal AgeI site found at 6902
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 965
    Illegal SapI site found at 4689
    Illegal SapI site found at 6884
    Illegal SapI.rc site found at 5607


Design Notes

Source

References